Organovo Holdings, Inc., a 3D biology company focused on delivering scientific and medical breakthroughs using its 3D bioprinting technology, has recently declared a publication in the scientific journal, PLOS One, which demonstrates the superiority of Organovo’s 3D bioprinted human liver tissues to effectively model drug-induced liver injury and distinguish between highly-related compounds with different toxicity profiles.
Using Organovo’s 3D bioprinted human liver tissues, researchers from Organovo and Roche Pharmaceutical Research and Early Development (“Roche”) were able to detect significant dose-dependent toxicity of trovafloxacin at clinically relevant doses, compared to levofloxacin, a structurally related, but non-toxic compound. Organovo’s 3D bioprinted human liver tissues are composed of patient-derived parenchymal (hepatocyte) and non-parenchymal (endothelial and hepatic stellate) cell populations. In addition, the Company’s 3D bioprinting technology creates tissues that are both spatially patterned and three-dimensional. This solution allowed researchers to perform histologic analyses in this study that revealed distinct intercellular hepatocyte junctions, CD31+ endothelial networks, and desmin-positive, smooth muscle actin-negative quiescent stellates. Unlike 2D cell cultures, Organovo’s tissues maintained metabolically relevant levels of ATP and albumin and drug-induced enzyme activity of cytochrome P450s for more than four weeks in culture.